Anti-Amyloid beta mAbs

Cat. No.

MABL-404

Name

Anti-Amyloid beta mAbs

Clone No.

AFD- scFv59

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, MTT assay, ThT assay, WB, ELISA, IHC

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody is specific for oligomeric as   well as fibrillar/aggregated Aβ.

Alternative Name

Aβ; Abeta; Amyloid-beta precursor protein; APP; ABPP; APPI; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide (CVAP); PreA4; Protease nexin-II (PN-II)

UniProt

P05067

Immunogen

The original antibody was isolated from a human scFv phage library by screening against Abeta1-42.

Application Notes

The scFv version of this antibody   demonstrated the highest ELISA titer for Aβimmunoreactivity against Aβ1–42,  and its specificity for oligomeric and fibrillar/aggregated Aβ was   demonstrated in a WB. The scFv version of this antibody reacted with amyloid beta   deposits in brain sections from a Tg2576 mouse and in skeletal muscle   sections from a Tg13592 mouse, as observed via IHC. The scFv version of this   antibody was used to inhibit the formation of Aβ fibril formation in vitro as   demonstrated in a Thioflavin T assay. This inhibition contributed to the   amelioration of Aβ cytotoxicity as was shown in vitro in an MTT assay; cell   viability increased from 45.2 ± 0.78% to 58.8 ± 5.45% (P = 0.069) in the   presence of scFv59. Furthermore, Tg2576 mice subjected to scFv59 injection in   the hippocampus and cortex had fewer diffuse and fibrillar Aβ deposits in those areas than Tg2576 mice injected with PBS (Fukuchi et al., 2006; PMID   16630540). Vascular amyloid was also stained by scFv59 (Fukuchi et al., 2006;   PMID  16766200).Antibody First   Published in  Fukuchi et al.

Antibody First Published

The original publication explores the   potential of a human single-chain antibody that

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.