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Anti-antibody heavy chain mAbs

简要描述:

This antibody is specific for the heavy chain chain of ferret B cell immunoglobulin.

产品参数:

Cat. No.:MABL-416

Species:Engineer

Species Reactivity:Ferret (Mustela putorius furo)

Type:Recombinate

Application:WB, ELISA, FC

详细介绍

Key features and details

Cat. No.

MABL-416

Name

Anti-antibody heavy chain mAbs

Clone No.

AFD- 11-E3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, FC

Species Reactivity

Ferret (Mustela putorius furo)

Basic Information

Specificity

This antibody is specific for the heavy chain chain of ferret B cell immunoglobulin.

Alternative Name

B cell immunoglobulin; ferret B Cell Heavy Chain; Ig gamma; IgG; ferret immunoglobulin; E3

UniProt


Immunogen

The original mouse IgG1 version of this antibody was raised against ferret immunoglobulins (IgG + IgM).

Application Notes

 This antibody is recommended for the   detection of ferret B cell immunoglobulin. It was screened for reactivity   against ferret peripheral blood mononuclear cells by FCM what demonstrated   specificity for CD79B+ B cells (Kirchenbaum and Ross, 2017; pmid28286781). It is   suitable for Western Blot, ELISA and Flow Cytometry applications.   Enzyme-linked immunosorbent assay with the murine version of this antibody  (ELISA) was used to assess the reactivity against purified ferret Ig and to determine the IgG subclass and concentration of the respective mAb (Kirchenbaum and Ross, 2017; pmid28286781). Further, a competitive ELISA was performed using unlabeled   and biotinylated MαF-Ig mAb to identify overlapping epitope recognition   (Kirchenbaum and Ross, 2017; pmid28286781). 

Antibody First Published

Generation of Monoclonal Antibodies against   Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo) J   Immunol Res. 2017;20175874572. PMID28286781

Note on publication


COA Information For reference only, actual COA shall prevail

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.


 


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