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Anti-Atrazine mAbs

简要描述:

This antibody binds organic plant herbicide atrazine.

产品参数:

Cat. No.:MABL-462

Species:Engineer

Species Reactivity:Species independent

Type:Recombinate

Application:ELISA

详细介绍

Key features and details

Cat. No.

MABL-462

Name

Anti-Atrazine mAbs

Clone No.

AFD- ATZ10#153

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Species independent

Basic Information

Specificity

This antibody binds organic plant herbicide atrazine.

Alternative Name

C8H14ClN5; 1912-24-9; Gesaprim; Atrazin; Chromozin

UniProt


Immunogen

The original antibody was generated  immunizing a BALB/c mouse with atrazine and then expressing the antigen   binding domain in a transgenic Nicotiana tabacum plant as an scFv with human C- kappa constant domain and a hexa-histidine tail.

Application Notes

This antibody is expressed as up to 0.014%   of soluble leaf protein and has a binding profile in ELISA, against an   atrazine-bovine serum albumin (BSA) conjugate. It is also reported to bind   free atrazine as well as related triazine herbicides (PMID: 9685621).

Antibody First Published

Longstaff et al. Expression and  characterisation of single-chain antibody fragments produced in transgenic   plants against the organic herbicides atrazine and paraquat. Biochim Biophys   Acta. (1998);1381(2):147-60. PMID:9685621

Note on publication

Describes the expression and characterization of this antibody in a transgenic plant.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.


 


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