Key features and details | |
Cat. No. | MABL-462 |
Name | Anti-Atrazine mAbs |
Clone No. | AFD- ATZ10#153 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds organic plant herbicide atrazine. |
Alternative Name | C8H14ClN5; 1912-24-9; Gesaprim; Atrazin; Chromozin |
UniProt | |
Immunogen | The original antibody was generated immunizing a BALB/c mouse with atrazine and then expressing the antigen binding domain in a transgenic Nicotiana tabacum plant as an scFv with human C- kappa constant domain and a hexa-histidine tail. |
Application Notes | This antibody is expressed as up to 0.014% of soluble leaf protein and has a binding profile in ELISA, against an atrazine-bovine serum albumin (BSA) conjugate. It is also reported to bind free atrazine as well as related triazine herbicides (PMID: 9685621). |
Antibody First Published | Longstaff et al. Expression and characterisation of single-chain antibody fragments produced in transgenic plants against the organic herbicides atrazine and paraquat. Biochim Biophys Acta. (1998);1381(2):147-60. PMID:9685621 |
Note on publication | Describes the expression and characterization of this antibody in a transgenic plant. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |