Anti-BNYVV Coat Protein mAbs

Cat. No.

MABL-530

Name

Anti-BNYVV Coat Protein mAbs

Clone No.

AFD- 14cG6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

dotblot, WB, ELISA

Species Reactivity

BNYVV

Basic Information

Specificity

Specific for epitope 4b (RTSP) which are amino acids 182-186 on the BNYVV coat protein. Binding is inhibited by large  concentrations of plant material, and the epitope is readily cleaved in the   presence of proteases.

Alternative Name

Beet necrotic yellowvein virus coat protein; BNYVV CP

UniProt

Q9YZK2

Immunogen

This antibody was raised by injecting purified BNYVV CP into mice.

Application Notes

This antibody is specific for epitope 4b of   the BNYVV coat protein. Its binding specificity has been confirmed in dot   blots (Commandeur et al, 1994) and western blots (Fecker et al, 1996) and   double antibody sandwich ELISAs using an alkaline-phosphatase fusion protein   (Uhde et al, 2000). It has been expressed as a scFv construct (Fecker et al,   1996) in both E. coli and higher plants.

Antibody First Published

Commandeur et. al. Location, Size, and   Complexity of Epitopes on the Coat Protein of Beet Necrotic Yellow Vein Virus   Studied by Means of Synthetic Overlapping Peptides Virology.1994 Jan;198(1)：282-7. PMID：7505070

Note on publication

Discusses the use of antibodies to analyse   the different epitopes of the BNYVV coat protein using antibodies which   recognise overlapping epitopes.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.