Anti-BNYVV P25 mAbs

Cat. No.

MABL-531

Name

Anti-BNYVV P25 mAbs

Clone No.

AFD- scFvP25

From

Recombinant Antibody

Isotype

Engineer antibody

Application

dotblot, ELISA

Species Reactivity

BNYVV

Basic Information

Specificity

Specifically binds to beet necrotic yellow vein   virus 25K protein but has some reactivity to sap from plants not infected   with BNYVV.

Alternative Name

P25; 25kDa Protein; Protein P25; 25 kDa non-structural protein; BNYVV 25kDa protein

UniProt

Q65669

Immunogen

This antibody was raised by injecting   purified P25 into mice.

Application Notes

This antibody is specific for the BNYVV   25kDa protein. Its binding spcificity has been confirmed in dot blots and   western blots (Fecker et al, 1996)

Antibody First Published

Fecker et al Expression of single-chain antibody fragments (scFv) specific for beet necrotic yellow vein virus coat protein or 25 kDa protein in Escherichia coli and Nicotiana benthamiana Plant   Molecular Biology 32： 979-986, 1996. PMID：8980548

Note on publication

Discusses the scFv fragment to test the   efficacy of PelB and PHA tags in higher plants on scFv fragments.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.