Key features and details | |
Cat. No. | MABL-532 |
Name | Anti-BoNT/A mAbs |
Clone No. | AFD- AR2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, inhibition, FC |
Species Reactivity | Clostridium botulinum |
Basic Information | |
Specificity | This antibody is specific for BoNT/A. |
Alternative Name | botA; BOTOX; Bontoxilysin-A; Botulinum neurotoxin type A1; Botulinum neurotoxin type A |
UniProt | P0DPI0 |
Immunogen | The original antibody was raised by mutating an antibody that was raised against two non- overlapping epitopes of BoNT/A. |
Application Notes | The binding of this antibody was determined using flow cytometry on BoNT/A1 and BoNT/A2. The structure of this antibody determined using x-ray crystallography (Garcia-Rodriguez et al., 2007; PMID：17173035). This antibody was used for a BoNT/A inhibition assay (Kalb et al., 2009; PMID：19399171). |
Antibody First Published | Garcia-Rodriguez et al. Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin Nat Biotechnol. 2007 Jan;25(1)：107-16. PMID：17173035 |
Note on publication | In the original paper they used yeast display and a co-selection strategy to increase cross-reactivity of a single chain (sc) Fv antibody to botulinum neurotoxin type A (BoNT/A). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
