Key features and details | |
Cat. No. | MABL-533 |
Name | Anti-Bordetella pertussis cells mAbs |
Clone No. | AFD- scFv 18 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Bordetella pertussis |
Basic Information | |
Specificity | This antibody is specific for pertactin and filamentous hemagglutinin of Bordetella pertussis. Filamentous hemagglutinin is a member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin FHA (fhaB). Pertactin may have a role in bacterial adhesion, and thus play a role in virulence. |
Alternative Name | filamentous hemagglutinin; FHA; pertactin; PRN; pertactin autotransporter; P.93; Pertactin; Filamentous hemagglutinin transporter protein FhaC; TpsB transporter; fhaC |
UniProt | P14283; P35077 |
Immunogen | This antibody was generated by immunizing a BALB/c mouse with 0.1 ml of the commercial pertussis vaccine Quandracel. From this DNA a phage display library was created and panned against B. pertussis whole cells so find this clone. |
Application Notes | The scFv version of this antibody was used for an ELISA on filamentous hemagglutinin (FHA), pertactin (PRN) and B. pertussis cells. The antibody was positive very every antigen tested. The scFv version of this antibody was tested for its ability to inhibit B. pertussis binding to HEp-2 cells, it did not show any inhibition. (Hussein et al, 2007; pmid：17724067). |
Antibody First Published | Hussein et al. Construction and Characterization of Single-Chain Variable Fragment Antibodies Directed against the Bordetella pertussis Surface Adhesins Filamentous Hemagglutinin and Pertactin. Infect Immun. 2007 Nov; 75(11)： 5476–5482. PMID: 17724067 |
Note on publication | A single-chain variable fragment (scFv) antibody library against Bordetella pertussis was constructed using M13 phage display. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
