Anti-Bordetella pertussis cells mAbs

Cat. No.

MABL-534

Name

Anti-Bordetella pertussis cells mAbs

Clone No.

AFD- scFv 5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assay, ELISA

Species Reactivity

Bordetella pertussis

Basic Information

Specificity

This antibody is specific for pertactin and   filamentous hemagglutinin of Bordetella pertussis. Filamentous hemagglutinin  is a member of a two partner secretion pathway (TPS) in which it mediates the   secretion of filamentous hemagglutinin FHA (fhaB). Pertactin may have a role   in bacterial adhesion, and thus play a role in virulence.

Alternative Name

filamentous hemagglutinin; FHA; pertactin;   PRN; pertactin autotransporter; P.93; Pertactin; Filamentous hemagglutinin   transporter protein FhaC; TpsB transporter; fhaC

UniProt

P14283; P35077

Immunogen

This antibody was generated by immunizing a   BALB/c mouse with 0.1 ml of the commercial pertussis vaccine Quandracel. From   this DNA a phage display library was created and panned against B. pertussis   whole cells so find this clone.

Application Notes

The scFv version of this antibody was used   for an ELISA on filamentous hemagglutinin (FHA), pertactin (PRN) and B.   pertussis cells. The antibody was positive very every antigen tested. The   scFv version of this antibody was tested for its ability to inhibit B.   pertussis binding to HEp-2 cells, normally only 35% of cells do not bind to   HEp-2 cells but this antibody increases that rate to 75%-100%. Furthermore,   the ability of the scFv version of this antibody to prevent B. pertussis   infection in mice. The viral load present in mice after 48h was a lot lower   than in normal circumstances (Hussein et al, 2007; pmid：17724067).

Antibody First Published

Hussein et al. Construction and   Characterization of Single-Chain Variable Fragment Antibodies Directed against   the Bordetella pertussis Surface Adhesins Filamentous Hemagglutinin and   Pertactin. Infect Immun. 2007 Nov; 75(11)： 5476–5482. PMID：17724067

Note on publication

A single-chain variable fragment (scFv)   antibody library against Bordetella pertussis was constructed using M13 phage   display.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.