Key features and details | |
Cat. No. | MABL-553 |
Name | Anti-Brg1 mAbs |
Clone No. | AFD-4E 5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | mAb 4E5 specifically recognises alpha-Brg1 |
Alternative Name | SMARCA4; alpha-BRG1; BAF190; BAF190A; BRG1; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; CSS4; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4 |
UniProt | P51532 |
Immunogen | Anti-Brg1 rat monoclonal antibodies were generated based on the rat lymph node method. An 8-week-old female lzm rat was injected via the hind footpads with 200 mL of emulsion containing 100 mg of recombinant mouse Brg1 protein and Freund’s complete adjuvant. After 3 weeks, the cells from the lymph nodes of a rat immunized with an antigen were fused with mouse myeloma Sp2/0-Ag14 cells at a ratio of 5 |
Application Notes | |
Antibody First Published | Ohkawa et al. Production of a Rat Monoclonal Antibody Against Brg1 HYBRIDOMA Volume 28, Number 6, 2009 PMID:20025509 |
Note on publication | Describes the role of BRG1 in the SWI/SNF complex as a DNA-dependent ATPase for chromatin remodelling, and mentions the need for a monoclonal antibody to distinguish BRG1 from BRM, which are highly similar. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
