Key features and details | |
Cat. No. | MABL-554 |
Name | Anti-BRIL mAbs |
Clone No. | AFD- BAG2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, ELISA |
Species Reactivity | Escherichia coli |
Basic Information | |
Specificity | The antibody is specific for BRIL. |
Alternative Name | Soluble cytochrome b562; Cytochrome b-562 |
UniProt | P0ABE7 |
Immunogen | The original antibody was isolated from a semi-synthetic phage display library of humanized Fab fragments, by panning against BRIL. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The crystal structure of the antibody in complex with BRIL was determined by cryoEM (Mukherjee et al., 2020; PMID: 32221310). |
Antibody First Published | Mukherjee et al., Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins. Nat Commun. 2020 Mar 27;11(1):1598. doi: 10.1038/s41467-020-15363-0. PMID:32221310 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
