Key features and details | |
Cat. No. | MABL-555 |
Name | Anti-Bromodeoxyuridine mAbs |
Clone No. | AFD- IIB5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, FC, IHC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The original antibody reacts with bromodeoxyuridine (BrdU) also when incorporated into nuclear DNA. Further, the antibody is known to cross-react with Iododeoxyuridine (IdU). |
Alternative Name | BrdU |
UniProt | |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse intraperitoneally with BrdU conjugated to Bovine Serum albumin. |
Application Notes | The binding characteristics of the antibody was determined in a radioimmunoassay using 3H-BrdUrd as a ligand, and the affinity constant was found to be 3.1 x 10^7 L/mol (Schutte et al., 1987; PMID: 3113895). This antibody was used for immunohistochemistry for the detection of bromodeoxyuridine (van Engeland et al., 1997; PMID: 9299167). |
Antibody First Published | Schutte et al. An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry Cytometry. 1987 Jul;8(4):372-6. doi: 10.1002/cyto.990080405. PMID:3113895 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
