Key features and details | |
Cat. No. | MABL-569 |
Name | Anti-C3a-receptor mAbs |
Clone No. | AFD- 2A1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the 2e loop of the human C3a receptor, amino acid residues 185-199 and 218–226 (TNDHPWTVP). |
Alternative Name | C3AR1; C3AR; C3a-R; Complement component receptor 1; C3a anaphylatoxin chemotactic receptor |
UniProt | Q16581 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with second extracellular loop of the C3aR (amino acid residues 173–332) fused to glutathione-S-transferase (GST). A combinatorial phage library was generated expressing scFv antibodies and panned against second extracellular loop of the C3aR, fused to the maltose binding protein as antigen. |
Application Notes | The original scFv antibody binds to the 2e loop of the human C3a receptor in an ELISA. Human C3a receptor is a G protein-coupled receptor stimulating chemotaxis, superoxide anion generation and granule enzyme release. This antibody was able to bind RBL-2H3 cells stably expressing the C3aR and to the human mast cell line HMC-1 in a flow cytometric assay. This antibody is not capable of blocking the interaction between C3a and C3aR (PMID： 11373092). |
Antibody First Published | Hawlisch et al. Site-specific anti-C3a receptor single-chain antibodies selected by differential panning on cellulose sheets. Analytica Biochemistry 2001; 293(1)：142-145.PMID：11373092 |
Note on publication | Describes the selection of scFv fragments with specificity for the C3a receptor from phage-display libraries. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
