Key features and details | |
Cat. No. | MABL-572 |
Name | Anti-C5a mAbs |
Clone No. | AFD- Fab 100 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | Fab 100 recognises both C5 and C5a. |
Alternative Name | Complement Component 5a; Complement C5; C3 and PZP-Like Alpha-2- Macroglobulin Domain Containing Protein 4; Complement Component 5; C5a Anaphylatoxin; Prepro-C5; CPAMD4; Anaphylatoxin C5a Analog; ECLZB; C5D; C5b |
UniProt | P01031 |
Immunogen | A panel of mAbs against the activated complement component C5a was obtained from a filamentous phage M13-Fab display library generated from mice immunized with human rC5a. Fabs isolated from the library after iterative selection against rC5a bound to both rC5a and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a but not on the native complement component C5, the library was rescreened in a competitive manner. The phage Fab library was first incubated with immobilized C5 to deplete C5 reactive Fabs. The C5 nonadherent phage were then incubated with immobilized rC5a in the presence of soluble C5. |
Application Notes | |
Antibody First Published | Ames et al. Isolation of Neutralizing Anti-C5a Monoclonal Antibodies from a Filamentous Phage Monovalent Fab Display Library Journal of Immunology, 1994, 152： 4572. PMID：8157971 |
Note on publication | Describes the generation of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
