Anti-C3b-iC3b mAbs

Cat. No.

MABL-571

Name

Anti-C3b-iC3b mAbs

Clone No.

AFD- 7C12

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, FC, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human C3b. C3 plays a central role in the activation of the complement system. Its   processing by C3 convertase is the central reaction in both classical and  alternative complement pathways. After activation C3b can bind covalently,   via its reactive thioester, to cell surface carbohydrates or immune   aggregates.

Alternative Name

Complement C3; C3; C3 and PZP-like   alpha-2-macroglobulin domain- containing protein 1

UniProt

P01024

Immunogen

The mouse version of this antibody was raised by immunizing a mouse with human C3b and iC3b bound to a carbohydrate matrix via the alternative pathway of complement activation.

Application Notes

To prepare and characterize this antibody,   an ELISA on iC3 protein was performed using the mouse version of this   antibody (Tosic et al, 1989; pmid：2786912). An immunoblot was performed on control, early AD, and AD   patient brain tissues using the mouse version of this antibody to determine the link between the complement system and Alzheimer’s disease (Wu et al,   2019; pmid：31433986). To study   the deposition of the complement protein fragment C3b and its breakdown products on CD20-positive cells, flow cytometry was performed on Raji or   ARH-77 cells using the mouse version of this antibody.

Antibody First Published

Wu et al. Preparation of monoclonal   antibodies to C3b by immunization with C3b(i)-sepharose J Immunol Methods. 1989 Jun 21;120(2)：241-9. PMID：2786912

Note on publication

Four monoclonal antibodies were prepared and   characterized to human C3b of high specificity and affinity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.