Key features and details | |
Cat. No. | MABL-595 |
Name | Anti-Calmodulin-domain protein kinase 1 mAbs |
Clone No. | AFD- VHH-1B7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, immunoprecipitation, ELISA |
Species Reactivity | Toxoplasma gondii |
Basic Information | |
Specificity | CDPKs regulate essential processes in parasites.VHH-IB7 recognises CDPK1 in a conformation- dependent manner and selectively inhibits the Ca^2+ bound form. It also recognises CDPK3 in a Ca2+- bound form. |
Alternative Name | CDPK1 |
UniProt | Q9BJF5 |
Immunogen | VHH phage display libraries from alpacas were pooled and panned against recombinant TgCDPK1, and from this clone 1B7 was selected. |
Application Notes | ELISA showed VHH-1B7 has reactivity against CDPK1. By immunoblot, 1B7 recognized both endogenous and recombinant versions of CDPK1 and its closest paralog CDPK3 (Ingram et al, 2015). Immunoprecipitation showed VHH-1B7 specifically recognises the Ca2+-bound form of CDPK1 and CDPK3. VHH-1B7 inhibits CDPK1 and CDPK3 with an IC50 of approximately 40 nm. The crystal structure of CDPK1 with VHH-1B7 showed VHH-1B7 interacts directly with the regulatory domain of CDPK1. |
Antibody First Published | Ingram et al, 2015. Allosteric activation of apicomplexan calcium- dependent protein kinases. Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):E4975-84 PMID:26305940 |
Note on publication | Describes the purification of VHH-1B7 from the phage library and its characterisation through ELISA, immunoprecipitation, and crystallography. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
