Key features and details | |
Cat. No. | MABL-604 |
Name | Anti-Capsid protein VP1 mAbs |
Clone No. | AFD- MabB |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, HI, NTRL, RIA, ELISA |
Species Reactivity | canine parvovirus, feline panleukopenia virus, Mink enteritis virus, Raccoon parvovirus |
Basic Information | |
Specificity | This antibody is specific for CPV-2's, FPV's capsid (site A). It also cross-reacts with MEV and RPV. |
Alternative Name | Coat protein VP1; CPV-2; FPV; MEV; RPV; ABCD_AD539 |
UniProt | P17455; P04864 |
Immunogen | The original antibody was generated by immunizing a Lewis-strain rat with purified infectious (heavy) FPV-b. |
Application Notes | The original version of this antibody (rat IgG2b) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV, FPV, and MEV-a and -b in neutralization and HI assays. It was also used in RIAs. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, FPV-a; -b; -c, RPV-a; -b; -c; -d; and -e, and MEV-a; -b; -c; -d; -e; -f; -g; and -h. In the RIA, it was found to react with CPV-d and FPV-b (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid:Fab complexes, and its Kd was estimated via ELISA as ~6.1 × 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structure was determined (Hafenstein et al., 2009; PMID: 19321620). |
Antibody First Published | Parrish & Carmichael Antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus. Virology. 1983 Sep;129(2):401-14. doi: 10.1016/0042-6822(83)90179-4. PMID:6194613 |
Note on publication | The original publication investigates the antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus, employing monoclonal antibodies to identify antigenic variants within and between virus isolates from different animal species. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
