Key features and details | |
Cat. No. | MABL-605 |
Name | Anti-Capsid protein VP1 mAbs |
Clone No. | AFD- Nano-25 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Cryo-EM, crystallography, ELISA |
Species Reactivity | Norwalk virus |
Basic Information | |
Specificity | This antibody is specific for the GII.10 group of the protruding (P) domain of the Norwalk virus capsid protein VP1. The P1 subdomain binding site is occluded on intact particles, yet this antibody binds intact norovirus VLPs and virions. |
Alternative Name | CP; Capsid protein; ABCD_AG266 |
UniProt | Q5F4T5 |
Immunogen | The original antibody was generated by immunizing a single alpaca with the GII.10 virus-like particle protein. |
Application Notes | The reactivity of the original antibody (alpaca VHH) against norovirus VLPs and (P) domains was determined using ELISA. Using isothermal titration calorimetry, a Kd of 28 nM was measured between the antibody and the GII.10 P domain. The crystal structure of this antibody in complex with norovirus GII.10 P domain was determined (Koromyslova & Hansman, 2015; PMID: 25520510). The epitope of this antibody was further characterized in X-ray crystallography and cryo-EM (Koromyslova & Hansman, 2017; PMID: 29095961). |
Antibody First Published | Koromyslova & Hansman, Nanobody binding to a conserved epitope promotes norovirus particle disassembly. J Virol. 2015 Mar;89(5):2718-30. doi: 10.1128/JVI.03176-14. PMID:25520510 |
Note on publication | The original publication characterizes Nano-25, a nanobody that specifically binds to a strain-specific epitope on human norovirus particles. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
