Key features and details | |
Cat. No. | MABL-617 |
Name | Anti-Carbonic anhydrase IX mAbs |
Clone No. | AFD- G10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, inhibition, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CA IX. |
Alternative Name | CA9; Carbonic anhydrase 9; Carbonate dehydratase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1 MN/CA IX; endogenous protein p54/58N; EC |
UniProt | Q16790 |
Immunogen | The original antibody was generated by panning a non-immune human scFv-phage display library against CA IX-expressing cells (293T-G250 cells). |
Application Notes | The specificity of this antibody's original format (human scFv) was validated via ELISA, demonstrating its selective binding to CA IX-expressing cells. It was further confirmed through flow cytometry and cross-competition studies. This antibody exhibited an IC50 of 2.086 µg/ml / 2.9 nM to stable G250-expressing 293T cells. This antibody was tested in vivo in Ncr Nude mice (athymic mice); it was incorporated into lentiviral vectors to create anti-CA IX chimeric immune receptors for T-lymphocyte transduction, resulting in enhanced T-lymphocyte cytotoxicity against renal cell carcinoma (RCC) cells. An scFv-Fc fusion version of this antibody was successfully used in an internalization study of anti-CA IX scFv- Fc antibody fusion proteins into stable G250-293T cells, exhibiting mild (~20%) inhibition (US8466263B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
