Key features and details | |
Cat. No. | MABL-616 |
Name | Anti-Carbonic anhydrase mAbs |
Clone No. | AFD- cAbCA05 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization |
Species Reactivity | Cow |
Basic Information | |
Specificity | The antibody is specific for carbonic anhydrase. |
Alternative Name | cAb-CA05; carbonic anhydrases; carbonate dehydratases; CA |
UniProt | P00921 |
Immunogen | The original antibody was generated by immunizing a dromedary with bovine erythrocytecarbonic anhydrase. A phage display library was constructed and the antibody was selected by panning. |
Application Notes | The crystal structures of a the VHH antibody both as free antibody (Decanniere et al., 2000; PMID: 10864500 and Desmyter et al. 2000; PMID: 11342547) and in complex with its antigen (Desmyter et al., 2000; PMID: 11342547) were determined. The VHH antibody recognized the bovine erythrocyte carbonic anhydrase with an affinity of 72 nM (Kd) (Desmyter et al., 2000; PMID: 11342547). The antibody did not inhibit the enzymatic activity of the antigen (Desmyter et al., 2000; PMID: 11342547 and Lauwereys et al., 1998; PMID: 9649422). |
Antibody First Published | Lauwereys et al. Potent enzyme inhibitors derived from dromedary heavy- chain antibodies. EMBO J. 1998 Jul 1;17(13):3512-20. PMID:9649422 |
Note on publication | The original paper describes the generation and characterization of the antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
