Key features and details | |
Cat. No. | MABL-626 |
Name | Anti-Cardiotin mAbs |
Clone No. | AFD- SR-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF |
Species Reactivity | Swine, Human |
Basic Information | |
Specificity | The original antibody is specific for cardiotin. Cardiotin is a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle. |
Alternative Name | |
UniProt | |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse with the 100 kDa cardiotin subunit. |
Application Notes | In immunofluorescence assays on cryostat sections of myocardium, the antibody showed the characteristic cardiotin staining pattern in cardiomyocytes. The antibody was reactive with the 60 kDa and the 100 kDa cardiotin subunits of the SR-enriched fraction of swine heart tissue in western blot analysis (Schaart et al., 1997; PMID:v 9042960). |
Antibody First Published | Schaart et al. Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein, FEBS Lett. 1997 Feb 17;403(2):168-72. PMID:9042960 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |