Key features and details | |
Cat. No. | MABL-647 |
Name | Anti-CCL20 mAbs |
Clone No. | AFD- AB1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, SPR, ELISA |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody is specific for murine CCL20. The antibody does not recognise human CCL20. |
Alternative Name | C-C motif chemokine 20; Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20 |
UniProt | O89093 |
Immunogen | |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. Capture ELISA was performed and the 1:1 binding kinetics and affinities were measured by surface plasmon resonance (Kd= 2.3 nM). CCR6 Chinese hamster ovary (CHO)-K1 β-arrestin G protein–coupled receptor functional assay was performed to measure the inhibition of the antibody (IC50= 30.7). |
Antibody First Published | Cannon et al. Experimentally guided computational antibody affinity maturation with de novo docking, modelling and rational design PLoS Comput Biol. 2019 May; 15(5): e1006980. PMID:31042706 |
Note on publication | The original paper describes the generation and computational affinity maturation of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
