Key features and details | |
Cat. No. | MABL-655 |
Name | Anti-CCR5 mAbs |
Clone No. | AFD- T21/8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | T21/8 antibody binds with high affinity to a CCR5 N-terminal epitope. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES. |
Alternative Name | CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor |
UniProt | P51681 |
Immunogen | This antibody was raised by immunizing mice with intact RBL-CCR5 cells. |
Application Notes | T21/8 antibody is recommedned for the detection and analysis of CCR5 via various methods. For instance, one group used this antibody to detect intracellular localization of CCR5 in RBL- CCR5 cells via immunofluorescence staining (Pollok-Kopp et al., 2003). Another group investigated ligand- mediated down-modulation of CCR5 via flow cytometry, utilizing T21/8 antibody (Fox et al., 2015). With other anti-CCR5 antibodies which react with CCR5 phosphorylated on different serine residues (Ab01578; Ab01579), it can be used to study dynamic regulation of CCR5 and phosphorylation mechanisms. |
Antibody First Published | Pollok-Kopp et al. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. Epub 2002 Oct 27. PMID:12403770 |
Note on publication | This article describes the generation and characterization of the T21/8 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
