Anti-CCR7 mAbs

Cat. No.

MABL-656

Name

Anti-CCR7 mAbs

Clone No.

AFD- 4B12

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assays, IP, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody was used for flow cytometry   and the staining quality can be improved by incubation at 37°C (Britschgi et   al, 2008). This antibody is widely used to monitor the activation of   dendritic cells in mice (Ohl et al, 2004; Ritter et al, 2004; Zhao et al,   2019). It was also utilized in immunoprecipitation of the CCR7 receptor   protein (Ritter et al, 2004). The antibody does not interfere with ligands   binding to the CCR7 receptor, which was confirmed by a transmigration assay   (Ritter et al, 2004). It does not interfere with T-cell migration (Ritter et   al, 2004). The antibody also is not agonistic as it does not activate the   receptor (Ritter et al, 2004).

Alternative Name

EBI1; EBV-induced G-protein coupled receptor   1; Epstein-Barr virus- induced G-protein coupled receptor 1; MIP-3 beta  receptor; CD197; C-C CKR-7; CC-CKR-7; C-C chemokine receptor type 7

UniProt

P47774

Immunogen

This antibody was raised by immunizing rats   with mouse CCR7 antigen.

Application Notes

This antibody is specific to the mouse CCR7   protein, which was confirmed by immunoprecipitation (Ritter et al, 2004).

Antibody First Published

Ritter et al. Analysis of the CCR7   expression on murine bone marrowderived and spleen dendritic cells. J Leukoc   Biol. 2004 Aug;76(2):472-6. Epub 2004 Jun 14. PMID:15197229

Note on publication

The article describes the analysis of CCR7   expression during the maturation of bonemarrow-dendritic cells

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.