Name
VTA1 Human
Cat. No.
MAG-5124
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
VTA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 331 amino acids (1-307 a.a.) and having a molecular mass of 36.5kDa. VTA1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms
Vacuolar protein sorting-associated protein VTA1 homolog, DRG-1, LYST-interacting protein 5, LIP5, SKD1-binding protein 1, SBP1, VTA1, C6orf55, DRG1My012, HSPC228.
Introduction
Vps20-associated 1 homolog (VTA1) is a member of the VTA1 family. The VTA1 protein is involved in the endosomal multivesicular bodies (MVB) pathway. Furthermore, VTA1 is interacts with CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1) and possibly CHMP3. VTA1 is believed to be a cofactor of VPS4A/B, which catalyzes membrane-associated ESCRT-III assemblies. In addition, VTA1 is involved in the sorting and down-regulation of EGFR and also in HIV-1 budding.
Physical Appearance
Sterile filtered colorless solution.
Formulation
VTA1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 200mM NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSH MAALAP LPPLPAQFKS IQHHLRTAQE HDKRDPVVAY YCRLYAMQTG MKIDSKTPEC RKFLSKLMDQ LEALKKQLGD NEAITQEIVG CAHLENYALK MFLYADNEDR AGRFHKNMIK SFYTASLLID VITVFGELTD ENVKHRKYAR WKATYIHNCL KNGETPQAGP VGIEEDNDIE ENEDAGAASL PTQPTQPSSS STYDPSNMPS GNYTGIQIPP GAHAPANTPA EVPHSTGVAS NTIQPTPQTI PAIDPALFNT ISQGDVRLTP EDFARAQKYC KYAGSALQYE DVSTAVQNLQ KALKLLTTGR E.
Safety Data Sheet
SDS
Usage
Mabioways products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
