Name
CST4 Human, sf9
Cat. No.
MAG-5271
Tag/Conjugates
His
Source
Sf9,
Baculovirus cells.
Shipped
Shipped with Ice Packs
Description
CST4 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 131 amino acids (21-141 a.a.) and
having a molecular mass of 15.4kDa (Migrates at 13.5-18kDa on SDS-PAGE under
reducing conditions). CST4 is expressed with a 10 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Synonyms
Cystatin
S, Salivary Acidic Protein 1, Cystatin-SA-III, Cystatin-4, Cystatin 4,
Cystatin-S.
Introduction
CST4 is a member of the cystatin superfamily which contains proteins that hold multiple cystatin-like sequences. Several family members are active cysteine protease inhibitors, whereas others have lost or possibly never attained this inhibitory activity. CST4 strongly inhibits papain (non-competitively) and ficin, partially inhibits stem bromelain and bovine cathepsin C, however does not inhibit porcine cathepsin B or clostripain.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
CST4 protein solution (1mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
ADP MSSSKEE NRIIPGGIYD ADLNDEWVQR ALHFAISEYN KATEDEYYRR PLQVLRAREQ TFGGVNYFFD VEVGRTICTK SQPNLDTCAF HEQPELQKKQ LCSFEIYEVP WEDRMSLVNS R CQE A HHHHH H .
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
