Name
SKP E. Coli
Cat. No.
MAG-5605
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
SKP Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 162 amino acids (21-161 a.a.) and having a molecular mass of 17.9 kDa. The SKP is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Synonyms
hlpA, ompH, Chaperone protein skp, skp.
Introduction
SKP is a 17kDa trimeric periplasmic chaperone that supports outer membrane proteins in their folding and insertion into membranes. SKP protein is necessary for the normal release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane.
Physical Appearance
Sterile filtered colorless solution.
Formulation
SKP E.Coli solution containing 20mM Tris-HCl pH-8 & 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH M ADKIAIVNM GSLFQQVAQK TGVSNTLENE FKGRASELQR METDLQAKMK KLQSMKAGSD RTKLEKDVMA QRQTFAQKAQ AFEQDRARRS NEERGKLVTR IQTAVKSVAN SQDIDLVVDA NAVAYNSSDV KDITADVLKQ VK.
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
