Name
H2AFZ Human
Cat. No.
MAG-6222
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
H2AFZ Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 151 amino acids (1-128 a.a) and
having a molecular mass of 15.9kDa. H2AFZ is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Synonyms
H2A Histone Family, Member Z, H2AZ, H2A/z, H2A.Z-1, H2A.z,
H2AZ Histone, Histone H2A.Z.
Introduction
Histone
H2A.Z (H2AFZ) belongs to the histone H2A family. Histones are basic nuclear
proteins which are responsible for the nucleosome structure of the chromosomal
fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped
around a histone octamer composed of pairs of each of the four core histones
(H2A, H2B, H3, and H4). H2AFZ is implicated in the formation of constitutive
heterochromatin and vital for chromosome segregation during cell division.
Moreover, variant Histone H2A, which takes the place of conventional H2A in a
subset of nucleosomes. Studies in mice have demonstrated that this particular
histone is essential for embryonic development and show that lack of functional
histone H2A leads to embryonic lethality.
Physical Appearance
Sterile Filtered clear solution.
Formulation
H2AFZ protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Purity
Greater
than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MAGGKAG KDSGKAKTKA VSRSQRAGLQ FPVGRIHRHL KSRTTSHGRV
GATAAVYSAA ILEYLTAEVL ELAGNASKDL KVKRITPRHL QLAIRGDEEL DSLIKATIAG GGVIPHIHKS
LIGKKGQQKT V
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
