Name
FGF 2 Human Protein
Cat. No.
MAG-1938
Tag/Conjugates
His
Source
Rice Grain (Oryza Sativa).
Shipping
At Room Temperature
Description
FGF-2 Human Recombinant produced in rice is a single, non-glycosylated polypeptide chain containing 146 amino acids and having a molecular mass of ~17kDa. The FGF-b protein is purified by proprietary chromatographic techniques.
Synonyms
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b, Fibroblast growth factor 2, Basic fibroblast growth factor, Heparin-binding growth factor 2.
Introduction
Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
FGF-b was lyophilized from a concentrated solution without any additives.
Stability
Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
The ED 50 , as calculated by the dose-dependent proliferation of Balb/c 3T3 cells expressing FGF receptors is < 1 ng/ml, corresponding to a specific activity of > 1 x10 6 Units/mg.
Amino acid sequence
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Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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Solubility
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O at 100µg/ml, which can then be further diluted to other aqueous solutions.
