Name
IGF2 Mouse Protein
Cat. No.
MAG-2072
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
At Room Temperature
Description
IGF2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, monomeric polypeptide chain containing 67 amino acids and having a total molecular mass of 7.4kDa. The IGF2 is purified by proprietary chromatographic techniques.
Synonyms
Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734
Introduction
Insulin-like growth factor II (IGF-II) is an important foetal growth hormone.IGF-II is made by theca cells during gestation. IGF-II binds the sink IGF-II receptor (IGF2R) which results in IGF-II degradation.IGF-II also engages the IGF-I receptor (IGF1R) to arbitrate embryonic growth.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a sterile (0.2µ) filtered solution containing 0.1 % trifluoroacetic acid (TFA).
Stability
Lyophilized IGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF2 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
The ED 50 as determined by FDC-P1 cell proliferation is ≤ 50 ng/mL, corresponding to a specific activity of ≥ 2.0 x 10^4 units/mg.
Amino acid sequence
AYGPGETLCG GELVDTLQFV CSDRGFYFSR PSSRANRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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Solubility
It is recommended to reconstitute the lyophilized IGF2 in sterile water at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.
