Name
PDGF CC Human Protein
Cat. No.
MAG-2177
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
At Room Temperature
Description
PDGF-CC Human Recombinant (235-345) produced in E.Coli is a disulfide-linked homodimer containing 2x118 amino acids and having a total molecular mass of 26.8kDa. The PDGF-CC is fused to a 7 amino acid His tag [M-HHHHHH] at N-terminal and purified by proprietary chromatographic techniques.
Synonyms
Platelet Derived Growth Factor C, Spinal Cord-Derived Growth Factor, FALLOTEIN, PDGF-C, VEGF-E, SCDGF, Secretory Growth Factor-Like Protein, Platelet-Derived Growth Factor C, PDGFC.
Introduction
Platelet Derived Growth Factor-CC (PDGF-CC) belongs to the PGDF family of growth factors. PDGF-CC binds with high-affinity to PDGF R-a and activates PDGF R-ab heterodimers. During development, PDGF-CC is involved in ductal morphogenesis, cardiovascular smooth muscle cell proliferation; also PDGF-CC is an angiogenic factor. Furthermore, PDGF-CC is expressed in numerous tumors and tumor cell lines, and may be linked with tumorigenesis.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.
Stability
Lyophilized PDGF-CC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-CC should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Biological Activity
The ED 50 , as measured in a proliferation assay using mouse NR6R-3T3 cells, is less than 350ng/ml.
Amino acid sequence
MHHHHHHVVD LNLLTEEVRL YSCTPRNFSV SIREELKRTD TIFWPGCLLV KRCGGNCACC LHNCNECQCV PSKVTKKYHE VLQLRPKTGV RGLHKSLTDV ALEHHEECDC VCRGSTGG.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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Solubility
It is recommended to reconstitute the lyophilized PDGF-CC in sterile 18M-cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
