Name
Prolactin Ovine Protein
Cat. No.
MAG-2217
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
At Room Temperature
Description
Prolactin Ovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa. The Prolactin n is purified by proprietary chromatographic techniques.
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Introduction
Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO 3 .
Stability
Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Biological Activity
Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Amino acid sequence
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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Solubility
It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H 2 O or 0.4% NaHCO 3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
