Key features and details | |
Cat. No. | MABL-3452 |
Name | Anti-Lag3 [C9B7W](MABL-3452) |
Clone No. | C9B7W |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, IP, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for an epitope in the D2 domain of murine LAG-3. |
Alternative Name | CD223; LAG3; Lymphocyte activation gene 3 protein |
UniProt | Q61790 |
Immunogen | This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein. |
Application Notes | This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild‐type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen‐induced IL‐2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015). |
Antibody First Published | Workman et al. Phenotypic analysis of the murine CD4‐related glycoprotein, CD223 (LAG‐3) Eur J Immunol. 2002 Aug;32(8):2255-63 PMID:12209638 |
Note on publication | Describes the original generation of this antibody, and its characterisation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
