Anti-CD115/M-CSFR [AFS98](MABL-3461)

Cat. No.

MABL-3461

Name

Anti-CD115/M-CSFR [AFS98](MABL-3461)

Clone No.

AFS98

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, WB, FC, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody recognises mouse M-CSFR (CD115, CSF-1R) which is a membrane protein that influences tissue macrophage and osteoclast differentiation and proliferation.

Alternative Name

CSF-1R; Macrophage colony-stimulating factor 1 receptor; Csf1r; CD115; CSF-1 receptor; EC 2.7.10.1; CSF-1-R; M-CSF-R; Proto-oncogene c-Fms

UniProt

P09581

Immunogen

This antibody was raised against CD115.

Application Notes

AFS98 has been widely used in various flow cytometric   analyses to label CD115 antigen. For instance, one group used it to detect   monocytes in a mouse blood sample (Buschor et al., 2019). Similarly, Huo and   colleagues (2017) utilised this antibody to identify monocytes in mouse blood   in order to assess whether myelopoiesis was not a cause of the increase in   the monocytes number. This antibody has been also successfully used to   deplete macrophages (Ho et al., 2016). Finally, it was also utilised in the   immunofluorescence staining of tissue sections prepared from 4%   paraformaldehyde-fixed and decalcified bones (Dong et al., 2016).

Antibody First   Published

Sudo et al. Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene. 1995 Dec 21;11(12):2469-76. PMID:8545103

Note on publication

This article describes the generation and characterisation of the AFS98 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.