Key features and details | |
Cat. No. | MABL-3462 |
Name | Anti-PD-L1 [10F.9G2](MABL-3462) |
Clone No. | 10F.9G2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ELISA, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine PD-L1. |
Alternative Name | CD274; PDL1; PD L1; Programmed cell death 1 ligand 1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; 10F9G2; 10F 9G2 |
UniProt | Q9EP73 |
Immunogen | This antibody was raised by immunising Lewis rats with murine PD-L1 cDNA and murine PD- L1 CHO transfectants. |
Application Notes | The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1 expression in frozen tissue sections of murine brain (Eppihimer et al, 2002) and murine hearts (Rodig et al, 2003). This antibody has been shown to block the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This antibody precipitates diabetes in NOD mice and in adoptive transfer models of CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011). |
Antibody First Published | Eppihimer et al. Expression and Regulation of the PD-L1 Immunoinhibitory Molecule on Microvascular Endothelial Cells Microcirculation. 2002 Apr;9(2):133-45. PMID:11932780 |
Note on publication | Describes the original generation of this antibody, and its use in ELISA, flow cytometry and immunohistochemistry. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
