Name
BD 3 Rat Protein
Cat. No.
MAG-1312
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
At Room Temperature
Description
BD-3 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.5kDa. The BD-3 is purified by proprietary chromatographic techniques.
Synonyms
Beta-defensin 3, BD-3, Defensin beta 3, Defb3.
Introduction
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103A, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense.
Biological Activity
Measured by its antimicrobial activity against E. coli. The ED 50 for this effect is typically 4-20µg/ml, corresponding to a specific activity of 50,000-250,000units/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
BD-3 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized BD-3 in sterile 18M-cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized BD-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino acid sequence
KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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