Name
CTLA4 Mouse Protein
Cat. No.
MAG-1377
Tag/Conjugates
His
Source
Sf9 Insect cells.
Shipping
Shipped with Ice Packs
Description
CTLA4 Mouse Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 363 amino acids (38-161aa) and having a molecular mass of 40.6kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). CTLA4 is fused to a 239 amino acid hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.
Synonyms
Cytotoxic T-lymphocyte
protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, Ctla4,
Cd152.
Introduction
CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with ins.-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Biological Activity
The ED50 range ≤150ng/ml and the activity is determined by the IL-2 ELISA in a using stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80.
Physical Appearance
Sterile filtered colorless solution.
Formulation
CTLA4 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Solubility
/
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
IQVTQPSVVL ASSHGVASFP CEYSPSHNTD EVRVTVLRQT NDQMTEVCAT TFTEKNTVGF LDYPFCSGTF NESRVNLTIQ GLRAVDTGLY LCKVELMYPP PYFVGMGNGT QIYVIDPEPC PDSD LEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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