Name
IL 1 alpha Rat Protein
Cat. No.
MAG-1460
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
Shipped with Ice Packs
Description
IL 1 alpha Rat Recombinant produced in E.Coli is a
single, non-glycosylated polypeptide chain containing 179 amino acids (115-270 a.a)
and having a molecular mass of 20.2kDa. IL 1 alpha is fused to a 23 amino acid His-tag at
N-terminus & purified by proprietary chromatographic techniques.
Synonyms
Interleukin-1 alpha, IL-1 alpha.
Introduction
IL-1 alpha is produced by activated macrophages, stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1A proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Biological Activity
The biological activity was Rat IL1 alpha was measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is less or equal to 20 pg/ml.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
IL 1 alpha protein solution (1mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Solubility
/
Stability
Store at 4°C if entire vial will be used within 2-4
weeks.
Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS SAPHSFQ NNLRYKLIRI VKQEFIMNDS LNQNIYVDMD RIHLKAASLN
DLQLEVKFDM YAYSSGGDDS KYPVTLKVSN TQLFVSAQGE DKPVLLKEIP ETPKLITGSE TDLIFFWEKI
NSKNYFTSAA FPELLIATKE QSQVHLARGL PSMIDFQIS.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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