Name
IL 7 Rat Protein
Cat. No.
MAG-1590
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
At Room Temperature
Description
IL 7 Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 15.0kDa. The IL 7 is purified by proprietary chromatographic techniques.
Synonyms
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
Introduction
IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.
Biological Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is less than 0.2ng/ml, corresponding to a specific activity of > 5,000,000IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized IL-7 in sterile 18M-cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized IL-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-7 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Amino acid sequence
DCHIKDKDGK AFGSVLMISI NQLDKMTGTD SDCPNNEPNF FKKHLCDDTK EAAFLNRAAR KLRQFLKMNI SEEFNDHLLR VSDGTQTLVN CTSKEEKTIK EQKKNDPCFL KRLLREIKTC WNKILKGSI
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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