Name
IL-17E Human Protein
Cat. No.
MAG-1619
Tag/Conjugates
His
Source
HEK293 cells.
Shipping
Shipped with Ice Packs
Description
IL-17E Human Recombinant produced in HEK293 cells is a single, non-glycosylated polypeptide chain containing 154 amino acids (33-177a.a) and having a molecular mass of 17.8kDa. IL-17E is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Synonyms
interleukin-25 isoform 1, interleukin 25, IL17E, IL-17E, IL25, IL-25, interleukin-17E, interleukin-25.
Introduction
IL-17Ealso called IL-25,has a sequence similarity with IL17. IL-17E indluces NF-kappaB activation, and stimulates the production of IL-8. IL17E and IL17B are ligands for the cytokine receptor IL17BR. IL-25 is a proinflammatory cytokine favoring Th2-type immune response. The upregulation of costimulation-induced IL-17E receptors and release of cytokines and chemokines from IL-17E treated costimulated Th cells are differentially regulated by intracellular JNK, p38 MAPK and NF-kappaB activity. Blocking Iinterleukin-25 prevents airway hyperresponsiveness, a critical feature of clinical asthma. IL25 produced by innate effector eosinophils and basophils increase the allergic inflammation by enhancing the maintenance and functions of TSLP-DC activated adaptive Th2 memory cells. Over expression of IL-25 up-regulates gene expression of Th2 cytokines and induces growth retardation, jaundice, and multiorgan inflammation in a transgenic mouse model. IL-25 contributes to the induction and maintenance of eosinophilic inflammation by acting on lung fibroblasts which supports the fact that IL-17E is an important factor in asthma pathophysiology. IL-17E operates by amplifying TH2 cell-mediated allergic airway inflammation but doesn’t induce allergic inflammation in vivo.
Biological Activity
Measured by its binding ability in a functional ELISA with Human IL-17 RB.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
IL-17E protein (0.5mg/ml) contains Phosphate-Buffered Saline (pH 7.4) and 20% glycerol.
Solubility
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Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
DGSYSHWPSC CPSKGQDTSE ELLRWSTVPV PPLEPARPNR HPESCRASED GPLNSRAISP WRYELDRDLN RLPQDLYHAR CLCPHCVSLQ TGSHMDPRGN SELLYHNQTV FYRRPCHGEK GTHKGYCLER RLYRVSLACV CVRPRVMG HH HHHH
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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