Name
IL1A Human Protein
Cat. No.
MAG-1637
Tag/Conjugates
His
Source
HEK293 cells.
Shipping
At Room Temperature
Description
Interleukin-1 alpha Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 18kDa due to glycosylation. The IL1A is purified by proprietary chromatographic techniques.
Synonyms
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Introduction
IL-1 alpha is produced by activated macrophages, stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1A proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Biological Activity
Assay # 1: The biological activity was measured by its
binding ability in a functional ELISA, the ED50 is typically 0.5-5 µg/ml. Assay # 2: The biological activity was determined by the
dose-dependent stimulation of the proliferation of mouse D10S cells, the ED50
is typically less than 1pg/ml, corresponding to a specific activity of >1x10 9 unit/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The IL-1A protein was lyophilized from a 0.2µm filtered
solution of PBS pH 7.4 with 10% trehalose as protectant.
Solubility
It is recommended to reconstitute the lyophilized IL1A in
deionized water to a stock solution of 0.5mg/ml.
Stability
Lyophilized IL-1A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Amino acid sequence
/
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
/
