Name
IL-2 Canine Protein
Cat. No.
MAG-1651
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
At Room Temperature
Description
Interleukin-2 Canine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 136 amino acids and having a molecular mass of 15.6kDa. IL-2 is purified by proprietary chromatographic techniques.
Synonyms
Interleukin-2, IL-2, T-cell growth factor, TCGF.
Introduction
IL2 is a secreted cytokine that is essential for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 and interleukin 7. The expression of IL2in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the exact expression of a single gene.
Biological Activity
The ED 50 , as determined by CTLL-2 cell proliferation is <5ng/ml corresponding to a specific activity which is ≥ 2.0 x 10^5 units/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a sterile (0.2µm) filtered solution containing 20 mM sodium bicarbonate, pH 8.5.
Solubility
It is recommended to reconstitute the lyophilized IL-2 in Sterile 10 mM sodium bicarbonate, pH 8.5 at 0.1 mg/mL, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-2 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MAPITSSSTK ETEQQMEQLL LDLQLLLNGV NNYENPQLSR MLTFKFYTPK KATEFTHLQC LAEELKNLEE VLGLPQSKNV HLTDTKELIS NMNVTLLKLK GSETSYNCEY DDETATITEF LNKWITFSQS IFSTLT.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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