Name
IL-4 Canine Protein
Cat. No.
MAG-1692
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
At Room Temperature
Description
Interleukin-4 Canine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 109 amino acids and having a molecular mass of 12.9kDa. IL-4 is purified by proprietary chromatographic techniques.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Introduction
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which contributes to various overlapping roles of this cytokine and IL13. STAT6, a signal transducer and activator of transcription plays a main role in mediating the immune regulatory signal of this cytokine. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are regulated co-ordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
Biological Activity
The ED 50 , as determined by TF-1 cell proliferation is <25ng/ml corresponding to a specific activity which is ≥ 4.0 x 10^4 units/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a sterile (0.2µm) filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Solubility
It is recommended to reconstitute the lyophilized IL-4 in sterile 18MΩ-cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-4 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MHNFNITIKE IIKMLNILTA RNDSCMELTV KDVFTAPKNT SDKEIFCRAA TVLRQIYTHN CSNRYLRGLY RNLSSMANKT CSMNEIKKST LKDFLERLKV IMQKKYYRH.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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