Cat. No.
MAK-0052
Product Name
Rat cTnI (plasma)
Storage
2-8°C
Species
Rat
Instructions
All reagents should be allowed to reach room temperature before use.
Introduction
Cardiac troponin-I (CTNI) is a component of the troponin complex that regulates muscle contraction. After cardiac injury, CTNI is released into the blood. Because it is expressed specifically in the heart it is an excellent biomarker of cardiac injury. In humans, CTNI levels peak 12-24 hours after injury, returning to baseline within 2-6 days. In mice, levels peak as early as 1 hour and return to normal within 1-3 days.
Principle Of The Assay
This assay is intended for use with plasma samples. The ELISA uses two different antibodies that recognize a relatively proteaseresistant epitope on CTNI. One is used for solid phase immobilization (microtiter wells). The second is conjugated to horse radish peroxidase (HRP) and used for detection. Plasma samples are first diluted with three volumes of plasma diluent. Standards and diluted samples are then incubated in the microtiter wells with HRP conjugate for one hour. This results in CTNI molecules being sandwiched between the immobilization and detection antibodies. The wells are washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If CTNI is present a blue color develops. Color development is stopped by addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of CTNI is proportional to absorbance and is derived from a standard curve.
