Cat. No.
MAK-0055
Product Name
Monkey cTnI (serum/plasma)
Storage
4°C
Species
Monkey
Instructions
All reagents should be allowed to reach room temperature before use.
Introduction
Cardiac troponin-I (CTNI) is a component of the troponin complex that regulates muscle contraction. After cardiac injury, CTNI is released into the blood. Because it is expressed specifically in the heart it is an excellent biomarker of cardiac injury. In humans, CTNI levels peak 12-24 hours after injury, returning to baseline within 2-6 days. In mice, levels peak as early as 1 hour and return to normal within 1-3 days.
Principle Of The Assay
The ELISA uses two different antibodies that recognize a relatively protease-resistant epitope on CTNI. One is used for solid phase immobilization (microtiter wells). The second is conjugated to horse radish peroxidase (HRP) and used for detection. Samples (serum or plasma) and standards (200 µl) are pipetted into the microtiter wells and incubated for 2 hours on a plate shaker. After washing the wells, 100 µl of diluent and 100 µl of HRP-conjugate is pipetted into the wells. The plate is incubated for one hour. During this step, CTNI, if present, is sandwiched between the immobilization and HRP-conjugated antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If CTNI is present a blue color develops. Color development is stopped by addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of CTNI is proportional to absorbance and is derived from a standard curve.
