Cat. No.
MAK-0076
Product Name
Pig H-FABP
Storage
4°C
Species
Pig
Instructions
All reagents should be allowed to reach room temperature before use.
Introduction
Fatty acid-binding proteins are approximately 15 kDa cytoplasmic proteins involved in fatty acid transport and metabolism. The isoform expressed in heart (H-FABP) is highly abundant, representing 10-20 mol % of total cytoplasmic protein. H-FABP is released into blood after cardiac injury and provides a sensitive biomarker of myocardial necrosis in several species. H-FABP is expressed in both cardiac and skeletal muscle. When using it as a cardiac biomarker, it is therefore important to rule out skeletal muscle injury. In the absence of cardiac injury, H-FABP can also be used as a biomarker of skeletal muscle damage.
Principle Of The Assay
The assay uses affinity purified H-FABP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rabbit H-FABP antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in H-FABP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If H-FABP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of H-FABP is proportional to absorbance and is derived from a standard curve.
