Cat. No.
MAK-0078
Product Name
Rat & Mouse Cardiac MLC-1
Storage
2-8°C
Species
Rat & Mouse
Instructions
All reagents should be allowed to reach room temperature before use.
Introduction
Myosin light chains are released into circulation following muscle injury and provide useful biomarkers of muscle damage. Myosin light chain-1 (MLC-1) is expressed as different but immunologically related isoforms in cardiac and skeletal muscle. The antibodies used in this ELISA kit are approximately ten times more sensitive toward cardiac MLC-1 (CMLC-1). The assay provides an excellent tool for assessment of cardiac injury when skeletal muscle injury can be excluded or controlled.
Principle Of The Assay
The CMLC-1 ELISA uses two different CMLC-1 monoclonal antibodies. One is used for solid phase immobilization (on the microtiter wells). The second is conjugated to horseradish peroxidase (HRP) and is used for detection. The sample is diluted with diluent as necessary and 100 µl aliquots of samples and standards are incubated in the microtiter wells for one hour. The wells are then washed and anti-CMLC-1 HRP conjugate is added and incubated in the wells for one hour. CMLC-1 molecules are thereby sandwiched between the solid phase and HRP-conjugated antibodies. After washing to remove unbound HRP conjugate a solution of tetramethylbenzidine (TMB), an HRP substrate, is then added to the wells and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped by addition of 1N HCl changing the color to yellow. The concentration of CMLC-1 is proportional to the absorbance at 450 nm and is derived from a standard curve.
