Rabbit anti-Beta-Endorphin Antibody

Name

Rabbit anti-Beta-Endorphin Antibody

中文名字

兔抗β内啡肽抗体

Description

The antibody produces a strong biotin-streptavidin/HRP staining at a 1/1000 – 1/2000 dilution in rat anterior pituitary.
Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of Beta-Endorphin. Pre-adsorption of the diluted antibody with 10-6M of the following substances had no effect on Beta-Endorphin labeling: methionine enkephalin, leucine enkaphalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Carp, Cat, Guinea Pig, Human, Mouse, Newt, Rat

Preabsorption Control

/

Alternate Names

Endorfin-beta; Endorphin-beta; proopiomelanocortin, anti-Beta-Endorphin

RRID

AB_572220

Immunogen

This information is proprietary to Mabioway

Gene Symbol

POMC

Entrez Gene ID

Entrez Gene: 101087223  Cat
Entrez Gene: 100720803  Guinea Pig
Entrez Gene: 5443  Human
Entrez Gene: 18976   Mouse
Entrez Gene: 24664  Rat

NCBI Gene Aliases

ACTH,BE,Pomc-1,Pomc1,alpha-MSH,alphaMSH,beta-MSH,gamma-MS

Sequence

60617-12-1

APPLICATION

Quality Control

The antibody produces significant indirect immunofluorescent staining and biotin-avidin/HRP staining at a 1/1,000–1/2,000 dilution in rat anterior pituitary. Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of b-Endorphin. Preadsorption of the diluted antibody with 10-6 M of the following substances had no effect on b-Endorphin labeling: methionine enkephalin, leucine enkephalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.

Tissue

Rat anterior pituitary

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.

Absorption Control

/

Sections

10 µm cryostat

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn/AV-HRP according to manufacturer’s directions.

Suggested Dilution

1/1,000–1/2,000 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.