Rabbit anti-Neuropeptide Y Antibody

Name

Rabbit anti-Neuropeptide Y Antibody

中文名字

兔抗神经肽Y抗体

Description

Neuropeptide Y (NPY) is a member of a regulatory peptide family and has a marked sequence homology with pancreatic polypeptide (PP) and peptide YY (PYY), which are other members of the family.  NPY is widely expressed in the nervous system and has been shown to be differentially expressed in inhibitory interneurons in the hippocampus in degenerative disease, as a powerful vasoconstrictor in the periphery, and increased expression of NPY in the hypothalamus correlates with food intake.
In the rat central nervous system, immunohistochemistry has found NPY-like cell bodies in the cortex, caudate-putamen, hypothalamus (arcuate nucleus), hippocampus, anterior westolfactory bulb, nucleus accumbens, amygdaloid complex and periaqueductal grey. NPY-like fibers and terminals are detected in high numbers in the bed nucleus of the stria terminalis, the peri- and paraventricular regions of the hypothalamus and thalamus and in discrete hypothalamic nuclei, particularly the suprachiasmatic nucleus.
Westerns are not recommended for the Neuropeptide Y antibody since the protein is too small to detect in gel.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Alpaca (Llama), Bird, Bubalus Bubalis (Water Buffalo), Bullfrog, Cat, Ewe, Ferret, Fish, Frog, Frog (Xenopus Laevis), Ground Squirrel, Guinea Pig, Hamster, Human,  Monkey, Mouse, Ovine (Sheep), Pig, Psetta Maxima (Flat Fish), Rat, Sheep, Snake, Tadpole, Toad, Turtle, Zebrafish

Preabsorption Control

Available at Sigma Aldrich, Catalog # N3266

Alternate Names

Pro-neuropeptide Y; Neuropeptide tyrosine; prepro-neuropeptide Y; PYY4, anti-NPY

RRID

AB_572253

Immunogen

This information is proprietary to Mabioway

Gene Symbol

NPY

Entrez Gene ID

Entrez Gene: 102414548     Bubalus Bubalis (Water Buffalo)
Entrez Gene: 101096968     Cat
Entrez Gene: 443508          Ewe
Entrez Gene: 101686990     Ferret
Entrez Gene: 779985          Frog
Entrez Gene: 101976112    Ground Squirrel
Entrez Gene: 100379621    Guinea Pig
Entrez Gene: 100758436    Hamster
Entrez Gene: 4852             Human
Entrez Gene: 574114         Monkey
Entrez Gene: 109648         Mouse
Entrez Gene: 397304         Pig
Entrez Gene: 24604           Rat
Entrez Gene: 102454441   Turtle
Entrez Gene: 30281          Zebrafish

NCBI Gene Aliases

/

Sequence

P01303

APPLICATION

Quality Control

In rat central nervous system, the antiserum has significant staining with a very low background at a 1/5,000 –1/10,000 dilution using the Biotin-Streptavidin/HRP detection method. All staining is blocked by preabsorption of the diluted antiserum with excess NPY. Cross reactivity experiments in which diluted NPY antiserum was absorbed with excess peptide YY, avian pancreatic polypeptide, B-endorphin, vasoactive intestinal peptide, cholecystokinin or somatostatin showed no affect in blocking the intensity of staining.

Tissue

Rat paraventricular nucleus, cortex and caudate putamen

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over 20-30 min.
• Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Bn/Av-HRP at dilutions recommended by the manufacturer

Suggested Dilution

1/5,000 –1/10,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles