Rabbit anti-Parvalbumin Antibody

Name

Rabbit anti-Parvalbumin Antibody

中文名字

兔抗小清蛋白抗体

Description

The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus, cortex, and hippocampus.
The antiserum has been characterized as specific to parvalbumin; please see reference listed below. Recommended primary dilution is 1/5,000 – 1/8,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique.

Clonality

Polyclonal

Isotype

IgG

Host

Guinea Pig; Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

 Parvalbumin alpha; Pva; Parvalbumin (calcium binding protein); PALB1, PC255L (Millipore Cat #), anti-parvalbumin

RRID

AB_572259

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Pvalb

Entrez Gene ID

Entrez Gene:  5816           Human
Entrez Gene: 697272        Monkey
Entrez Gene: 19293          Mouse
Entrez Gene: 102094134   Pigeon
Entrez Gene: 25269           Rat

NCBI Gene Aliases

/

Sequence

P20472

APPLICATION

Quality Control

The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat thalamus, cortex, and hippocampus. The antiserum has been characterized as specific to parvalbumin; please see reference listed below.

Tissue

Rat thalamus, hippocampus and cortex

Perfusion Fixation

• Fixative - 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post fixation - 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn/AV-HRP according to manufacturer’s directions.

Suggested Dilution

1/5,000–1/10,000 in PBS/0.3% Triton X-100 - Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles