Rabbit anti-SP-1 Chromogranin A (Bovine) Antibody

Name

Rabbit anti-SP-1 Chromogranin A (Bovine) Antibody

中文名字

兔抗SP-1嗜铬粒蛋白A（牛）抗体

Description

The antibody has a proven strong staining at a 1/1000 – 1/2000 dilution in rat adrenal medulla using Biotin-Streptavidin/HRP detection method.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Bird (Agapornis Roseicollis), Bubalus Bubalis (Water Buffalo), Calves, Cat, Cattle, Chicken, Dog, Duck, Ewe, Fish, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Ostrich, Pig, Rat, Sheep

Preabsorption Control

/

Alternate Names

Pituitary secretory protein I; CgA; chromogranin A (parathyroid secretory protein 1), anti-SP-1 Chromogranin A

RRID

 AB_572227

Immunogen

This information is proprietary to Mabioway

Gene Symbol

CHGA

Entrez Gene ID

Entrez Gene: 102413232   Bubalus Bubalis (Water Buffalo)
Entrez Gene: 101087412   Cat
Entrez Gene: 281070         Cattle
Entrez Gene: 423420         Chicken
Entrez Gene: 607527         Dog
Entrez Gene: 101790234    Duck
Entrez Gene: 101114907    Ewe
Entrez Gene: 100717887    Guinea Pig
Entrez Gene: 100766424    Hamster
Entrez Gene: 100033828    Horse
Entrez Gene: 1113             Human
Entrez Gene: 574388         Monkey
Entrez Gene: 12652           Mouse
Entrez Gene: 104153770    Ostrich
Entrez Gene: 397540         Pig
Entrez Gene: 24258           Rat

NCBI Gene Aliases

/

Sequence

P10645

APPLICATION

Quality Control

The antibody has significant staining at a 1/1,000–1/2,000 dilution in rat adrenal medulla using Biotin/Avidin-HRP detection method.

Tissue

Rat adrenal medulla

Perfusion Fixation

• Fixative - 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20-30 min.
• Post Fixation - 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.

Absorption Control

Sections

10 µm cryostat

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Bn-AV/HRP according to manufacturer’s directions.

Suggested Dilution

1/1,000–1/2,000 in PBS/0.3% Triton X-100 - Bn-AV/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles